Chemistry Reference
In-Depth Information
Appendix A
Materials and Methods
This describes the various protocols and recipes used in this work.
Monoolein stock
I use 25mM monoolein in squalane as my continuous phase.
Molecular weight of monoolein: 356.64
Tomake 25mMof monoolein in squalane I use 89.16mg in 10mL (i.e. 8.916mg/mL)
Monoolein/Cholesterol stock
A mixture of 25mM monoolein and 25mM cholesterol in squalane is used.
Molecular weight of cholesterol: 386.65
Tomake 25mMof cholesterol in squalene I use 96.6625mg in 10mL (i.e. 9.7mg/mL)
Gramicidin
10
g/mL of gramicidin A in ethanol is used which is added to the electrolyte
(Sect.
A
). Molar mass of gramicidin is 1882.3g/mol. This means that the molar-
ity is approximately 5
µ
.
5
µ
M.
