Synopsis - Chlorophyll Biosynthesis and Technological Applications - page 52

Fig. 2.11 DV and MV
Chlide b
2.10.1.7 Discovery of Monovinyl Chlorophyllide
b Occurrence in Green Plants
Over the years, the origin of Chl b had been the subject of many debates. Shlyk
championed the idea that Chl b was formed from newly formed Chl a (Shlyk 1971 ).
On the other hand, Oelze-Karow and Mohr, suggested that Chl b was formed from
Chlide a (Oelze-Karow and Mohr 1978 ).
Then in 1981 and 1982, Duggan and Rebeiz, reported the detection of a MV
Chlide b pool in green plants (Duggan and Rebeiz 1981 , 1982c ) (Fig. 2.11 , II). The
MV Chlide b chemical structure was ascertained by chemical derivatization cou-
pled to 77 K spectrofluorometry. The ubiquitous detection of MV Chlide b in green
plants indicated that the biosynthesis of MV Chl b most probably involved MV
Chlide b . The involvement of MV Chlide b in the biosynthesis of MV Chl b will be
discussed in Chaps. 12 and 13 .
2.10.1.8 Discovery of Divinyl Chlorophyll b Occurrence
in the Corn Nec 2 Mutant
The unambiguous occurrence of DV Chl b in the Corn Nec 2 mutant was reported
by Brereton et al. and was characterized by mass spectroscopy (Brereton
et al. 1983 ). It was characterized by Wu and Rebeiz ( 1985 ) by NMR and mass
spectroscopy. The metabolism of DV Chl b will be discussed in Chaps. 12 and 13 .