Some Major Steps in the Understanding of the Chemistry and Biochemistry of Chl - Chlorophyll Biosynthesis and Technological Applications - page 24

1.3.2 Rimington and Sveinsson, 1950
In 1950, Rimington and Sveinsson developed a spectrophotometric method for the
determination of uroporphyrin (Uro) (Rimington and Sveinsson 1950 ).
1.3.3 Koski, 1950
In 1950, Koski, derived simultaneous equations for the determination of Pchl
(ide) and Chl a and b by absorbance spectroscopy, in unsegregated plant extracts
(Koski 1950 ).
1.3.4 Nicolas and Rimington, 1951
In 1951, Nicolas and Rimington completed the development of a paper chro-
matographic technique for the separation of porphyrins with 2-8 carboxylic groups.
1.3.5 Dresel and Falk, 1956
In 1956, Dresel and Falk developed a method for the separation of Uro, Copro and
Proto, by solvent partition (Dresel and Falk 1956 ).
1.3.6 Seliskar, 1966
In 1966, Seliskar developed a thin-layer chromatographic technique for the separa-
tion of fully esterified and monocarboxylic tetrapyrroles.
1.3.7 Rebeiz, 1975
In 1975, Rebeiz et al. developed spectrofluorometric techniques for the detection
and quantitative determination of Proto and Pchl(ide) a formation in greening
tissues (Rebeiz et al. 1975a ).