Biology Reference
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Table 17.9 Tetrapyrrole accumulation and ALA content of washed morningglory leaf sections of
various ages following plant treatment with ALA and thioflavin T (0.5 lbs of thioflavin T and/or
2lbs per acre of ALA)
Accumulation after 15 h-incubation
ALA
Mp(e)
Pchlide
a
Age (days)
Treatment
nmoles per g fresh weight
Injury (%)
10
Control
0.2
0.1
0.0
0.0
2.7
1.2
0.0
0.0
2 ALA
0.3
0.1
2.2
2.1
17.8
7.1
95.6
8.7
0.5 TFT
0.2
0.1
0.0
0.0
2.3
1.1
0.0
0.0
2 ALA + 0.5 TFT
0.3
0.1
1.0
0.7
16.7
5.1
100
0.0
2 TFT
0.1
0.0
0.0
0.0
3.1
0.9
4.2
0.2
2 ALA + 2 TFT
0.2
0.1
3.3
4.2
22.0
19.5
87.5
16.0
15
Control
0.1
0.0
0.0
0.0
0.9
0.4
0.0
0.0
2 ALA
0.2
0.1
0.9
0.8
10.3
7.7
75.0
21.5
0.5 TFT
0.2
0.0
0.0
0.0
1.3
1.5
0.0
0.0
0.2
0.0
1.0
0.8
10.6
3.0
87.5
8.3
2 ALA + 0.5 TFT
0.1
0.1
0.0
0.0
1.7
0.4
20.9
25.1
2 TFT
2 ALA + 2 TFT
0.2
0.0
0.5
0.2
9.8
3.4
27.5
8.3
20
Control
0.1
0.0
0.0
0.0
0.5
0.3
0.0
0.0
2 ALA
0.2
0.1
0.1
0.1
3.2
2.7
52.8
5.7
0.5 TFT
0.1
0.1
0.0
0.0
0.8
0.6
12.6
16.1
2 ALA + 0.5 TFT
0.3
0.1
0.2
0.1
5.1
0.9
55.7
6.6
2 TFT
0.1
0.0
0.1
0.0
1.1
2.7
21.0
22.4
2 ALA + 2 TFT
0.2
0.0
0.1
0.1
3.8
2.4
50.2
22.4
LSD
ALA
¼
0.0
Pchlide
a
¼
3.2
Age
Mp(e)
¼
0.7
Injury
¼
1.0
LSD
ALA
¼
0.1
Pchlide
a
¼
4.6
Treatment
0.1
Values are means of four replicates. Photoperiodic damage was assessed 2 days after treatment.
TFT
Thioflavin T
Mp(e)
¼
0.9
Injury
¼
Fig. 17.7 Thioflavin T
The results reported in Table
17.9
, and Fig.
17.8
, confirmed that thioflavin T was
not an inducer of tetrapyrrole biosynthesis since in the absence of added ALA it did
not trigger an enhancement of tetrapyrrole biosynthesis (Rebeiz et al.
1990
).
Although thioflavin T slightly improved the penetration of ALA to inner tissue as
evidenced by detection of higher amounts of ALA in thioflavin T + ALA treated
tissues, the effect was marginal and for all practical purposes insufficient. So were
the effects on tetrapyrrole accumulation as evidenced by nearly equal amounts of
Pchlide
a
accumulation in the presence and absence of thioflavin T (Table
17.9
,
Fig.
17.8
). Photodynamic damage appeared to parallel tetrapyrrole accumulation as
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