Photodynamic Herbicides - Chlorophyll Biosynthesis and Technological Applications - page 386

Table 17.6 Tetrapyrrole accumulation and ALA content of grenhouse-grown whole
morningglory leaves of various ages following ALA treatment (2 lbs per acre)
ALA content and tetrapyrrole accumulation after 15 h dark-incubation

10-day old 15-day old 20-day old

C T C T C T

Tetrapyrrole or %
injury nmoles per g fresh weight

ALA 0 0 1.6 0.9 0.1 0 1.4 0.2 0.1 0.1 1.9 1.5

Mp(e) 0.1 0 2.4 2.4 0 0 4.7 6.3 0.1 0.1 1.7 2.2

Pchlide a 2.2 0.5 24.3 4.6 1.6 0.5 22.6 13.3 0.7 0.6 4.5 0.7

% Injury 0 0 96.3 5.4 0.0 0 83.8 2.1 0 0.0 72.9 12.7

LSD (0.05) age ALA ¼ 0.4

Mp(e) ¼ 2.8

Pchlide a ¼ 6.6

Injury ¼ 0.5

LSD (0.05) treatment ALA ¼ 0.4

Mp(e) ¼ 2.2

Pchlide a ¼ 5.4

0.4
Values are means of six replicates. Photoperiodic damage was assessed 10 days after treatment
(Reproduced from Kulur 1996 )

Injury ¼

Whole Leaf Analysis

70 220

CALA TALA CMp(e) TMp(e) CPide TPide

59

182

= LSDA ALA

49

CInj

TInj

= LSDA Mp(e)

143

= LSDA ALA

= LSDA Pide
= LSDA Inj

38

= LSDT Mp(e)
= LSDT Pide
= LSDT Inj

105

27

67

16

28

6

-5 -10

10 15 20 25

Plant Age (Days)

Fig. 17.4 ALA content, tetrapyrrole accumulation and photodynamic injury in unwashed whole
morningglory leaves of different ages. The data was adapted from Table 17.4 . C control, T treated,
ALA -aminolevulinic acid, Mp(e) Mg-Proto and/or its methyl ester, Pide Protochlorophyllide a ,

Inj photodynamic injury, LSD least significant difference at the 5 % level. Vertical graph bars refer
to standard deviation. Text bars refer to LSD magnitudes (Reproduced from Kulur 1996 )

δ