Biology Reference
In-Depth Information
16.6.1.2 Arabidopsis Mutants
A large number of mutants with defects in or elimination of chlorophyll/protein
complexes in
Arabidopsis thaliana
has already been identified (H. Bohnert,
personal communication). Also some interesting mutants can be obtained from
the US or European seed banks.
16.6.1.3 Lower Plant Mutants
•
Y-
1 mutant of
Chlamydomonas reinhardtii
(Gershoni et al.
1982
; Hoober
1990
;
Ish-Shalom and Ohad
1983
).
• Gr1BSL, G1BU, and O4BSL of
Euglena gracilis
(Cunningham and Schiff
1986
)
• PS I-less/
apc
E
Synechocystis
sp. PCC 6803 mutant (Shen and Vermaas
1994
)
16.6.2 Preparation of Photosynthetic Particles
A large volume of literature dealing with the preparation of various photosynthetic
particles is readily available. The most recent review of various procedures for the
preparation of PS I, PS II, LHCII, and a variety of smaller Chl-protein complexes
has been reported in (Paulsen and Scmid
2002
). Procedures described there can be
complemented by original standalone procedures available in the photosynthesis
literature.
16.6.3 Determination of Biosynthetic Routes Functional
in a Specific Mutant or Photosynthetic Particle
Partial and full biosynthetic routes that are functional in various mutants and isolated
photosynthetic particles and complexes can be determined by various techniques
described in (Rebeiz
2002
,
2003b
). These techniques have been developed over a
period of three and half decades and are routinely used on daily basis by several
scientists. Cold and
14
C-substrated can be prepared and used as described by (Rebeiz
2002
). Wild types and mutants can be light or dark adapted in order to poise them in
the DV or MV modes (Carey et al.
1985
) prior to subplastidic particle isolation.
Single or multistep reaction sequences can be executed by feeding appropriate
substrates in well defined cofactor media capable of supporting nearly all the
reactions described in Fig.
16.3
.
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