Biology Reference
In-Depth Information
16.6 Guidelines and Suggestions to Bioengineer Plants
with Smaller Photosynthetic Unit Size
The compatibility of the MBP-sublocation model of Chl-thylakoid protein assembly
has opened the way for testing the hypothesis of whether certain Chl biosynthetic
routes are indeed involved in the formation of specific Chl-protein complexes.
Below are outlined some guidelines and suggestions for investigating this issue.
The experimental strategy involves a two-pronged experimental approach.
In a first attempt, a variety of higher and lower plant mutants that lack specific
Chl-protein complexes could be used to determine which specific Chl biosynthetic
route(s) is/are missing from the mutant Chl biosynthetic pathway. In this manner it
may be possible to link a particular Chl biosynthetic route to a specific Chl-protein
complex formation.
Likewise in the second approach functional PS I, and PS II particles as well as
LHCII preparations could be isolated from wild types and mutants using mild
detergents and the putative Chl biosynthetic routes associated with a particular
preparation could be determined. In this manner it may be possible to link particular
Chl biosynthetic routes to the lateral heterogeneity of the PSU.
16.6.1 Selection of Mutants
A literature search of higher and lower plant mutants deficient in specific Chl-protein
complexes revealed a rather large number of such mutants. Final selection of specific
mutants for specific studies will therefore depend on the nature of the missing
Chl-protein complexes and availability of plant material. Below are listed some of
the candidate mutants
16.6.1.1 Mutants of Higher Plants Other Than Arabidopsis
•
chlorina-f2 viridis-m
29
viridis-n
34
and viridis-zd
69
of Barley (Henry et al.
1983
;
Machold et al.
1979
; Preiss and Thornber
1995
; White and Green
1987
).
• Chl
b
-less barley mutant (Bellemare et al.
1982
; Mullet et al.
1980
).
•
viridis-zb
63 and
viridis-h
15 of barley (Hiller et al.
1980
).
• Qy/+ hcf3/hcf3 of maize (Polacco
1984
).
•
hcf*
-3 nuclear maize mutant (Leto et al.
1985
).
• hcf1-2-3-6-19- 38-42-44-50-101-102-103-104-108-111 in maize (Miles
1994
).
• U374 mutant of sweet clover (Markwell et al.
1985
).
•C
ab4Bst
EII, C
ab4.23,
and C
ab4.3
mutants of tomato (Huang et al.
1992
).
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