The Chl a Carboxylic Biosynthetic Routes: (Photo) Conversion of Protochlorophyllides (Pchlides) a to Chlorophyllide (Chlide) a - Chlorophyll Biosynthesis and Technological Applications - page 244

Fig. 9.6 Formation of MV Chlide a by vinyl reduction of DV Chlide a via routes 4 and 8 in
DDV-LDV-LDDV plants (Adapted from Fig. 6.3 of Chap. 6 )

membrane-bound, NADPH-dependent enzyme (Parham and Rebeiz 1992 ; Parham
and Rebeiz 1995 ). It is a stable enzyme that has been solubilized and purified about
20 fold (Kolossov and Rebeiz 2001 ).
Discussion of Biosynthetic routes 4 and 8 in DDV-LDV-LDDV plants is given
below.

9.4.5.1 Formation of MV Chlide a by Vinyl Reduction of DV Chlide

a via Route 4 in Etiolated DDV-LDV-LDDV Plants in Darkness,

and in Greening DDV-LDV-LDDV Plants During the Initial
Dark Phases of the Photoperiod

The most spectacular manifestation of MV Chlide a formation is via route 4 is in
DDV-LDV-LDDV etiolated tissues induced to accumulate DV Pchlide a exclu-
sively, by successive dark-light treatments (Duggan and Rebeiz 1982a ). The
accumulated DV Pchlide a is photoconverted by a short light flash to DV Chlide
a and the latter is very rapidly converted to MV Chlide a in vivo and in organello
(Duggan and Rebeiz 1982b ).