The Chl a Carboxylic Biosynthetic Routes: Protochlorophyllide a - Chlorophyll Biosynthesis and Technological Applications - page 228

(MW 300,000) was described by Schopfer and Siegelman ( 1968 ). The purified

Pchl(ide) a -H exhibited a red absorption maximum at 639 nm, which also did not
reflect the spectral heterogeneity observed in vivo. In the light the red absorption
maximum shifted to a Chlide a red absorption maximum at 678 nm, which drifted to
672 nm in darkness. More purified Pchl(ide) a-Hs were prepared from etiolated barley
(MW 63,000) and bean (MW 100,000) by Henningsen and Kahn ( 1971 ).
Photoconversion yielded a Chl(ide) a complex with a red absorption maximum at
678 nm. In this case too, the spectral properties of the purified Pchl(ide) a -H did not
reflect the spectral heterogeneity observed in vivo.

¼

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