Biology Reference
In-Depth Information
Table 3.13 Reliability of Eqs. (
3.81
), (
3.82
) and (
3.83
) in the determination of the amount of
2-MV Pchl(ide)
b
in the presence of 2-MV Chl(ide)
b
Amount of pigment
added (pmol/ml)
Amount of 2-MV
Pchl(ide)
b
calculated
(pmol/ml)
Percentage error between
amounts added and
calculated (% )
Mean percentage
error
SD
(%
%) (%
%)
Chl(ide)
b
Pchl(ide)
b
482.4
96.9
85.3
12.0
2.7
10.3
265.3
161.4
143.9
10.8
289.4
161.4
133
17.6
337.7
161.4
186.8
15.7
385.9
161.4
172.7
7.0
410.0
161.4
153.5
4.9
434.12
161.4
154.1
4.5
458.2
161.4
164.8
2.1
530.6
161.4
162.5
0.7
2. Two fluorescence excitation spectra were recorded at 77
K, at emission
wavelengths of 660 and 643 nm.
3. The net fluorescence excitation amplitudes at 455 and 463 nm respectively, for
the 2-MV Chl(ide)
b
and 2-MV Pchl(ide)
b
components were calculated using
Eqs. (
3.81
) and (
3.82
). The apparent (E455 F6660)/(E463 F643) fluorescence
excitation ratio amounted to: 0.4210/0.3849
1.0938.
4. The molar ratio, of 2-MV Chl(ide)
b/
2-MV Pchl(ide)
b
was calculated from the
apparent (E455 F660)/(E463 F643) fluorescence excitation ratio with Eq. (
3.83
).
It amounted to 2.6707.
5. The calculated amount of 2MV-Pchl(ide)
b
in the mixture (153.5 pmol/ml) was
determined from the relation:
¼
2-MV Chl
ð
ide
Þ
b
=
2-MV Pchl
ð
ide
Þ
b
¼
2
:
6707
where 2-MV Chl(ide)
b
, as determined by fluorescence excitation at 293
K
amounted to 410.0 pmol/ml
3.9 Kinetic Analysis of Precursor-Product Relationships
in Complex Biosynthetic Pathways
tools that enable the determination of precursor-product relationships among
intermediates of irreversible but interconnected biosynthetic routes (Rebeiz
et al.
1988
). In an attempt to resolve this question, we have developed kinetic
equations that address this issue.
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