Biology Reference
In-Depth Information
3.8.2.2 Calculation of the 2-MV Pchl(ide) b/2-MV Chl(ide) b
Molar Ratio in a Mixture of Both Compounds
This procedure involved:
(a) calculation of the net fluorescence excitation amplitudes at 455 and 463 nm of
2 MV Chl(ide) b and 2-MV Pchl(ide) b respectively in a mixture of both
compounds, and
(b) calculation of the 2-MV Pchl(ide) b /2-MV Chl(ide) b molar ratio of the mixture
from the calculated net fluorescence signals.
Let the 463 nm excitation amplitude of the 2-MV Pchl(ide) b and 2-MV Chl(ide)
b mixture that is recorded at an emission wavelength of 643 nm in diethyl ether at
77 K be referred to as (E463 F660). Likewise let the 455 nm excitation amplitude
of the same mixture which is recorded at an emission wavelength of 660 nm, be
referred to as (E455 F660). Also, let X (4) in Eq. ( 3.37 ) represent 2-MV Pchl(ide)
b and Y in Eq. ( 3.38 ) represent 2-MV Chl(ide) b. By substituting (E463 F643) for
( E a F b ), (E455 F660) for ( E c F d ), 2-MV Pchl(ide) b for X and 2-MV Chl(ide) b for
Y ,Eqs.( 3.37 ) and ( 3.38 ) transform into:
2-MV Pchl
ð
ide
Þ
b
ð
E463 F643
Þ¼ ð
½
E463 F643
Þð
E455 F660
Þ=
k2
ð
1
=
K1
Þ
(3.79)
2-MV Chl
ð
ide
Þ
b
ð
E455 F660
Þ¼ ð
½
E455 F660
Þð
E463 F643
Þ=
k4
ð
1
=
K2
Þ
(3.80)
Where K 1 , K 2 and k 1 ,k 2 ,k 3 ,k 4 are as defined by Eqs. ( 3.39 ) and ( 3.40 ). By
substitution for X, Y, (E a F b ), and (E c F d ), Eq. ( 3.40 ) transforms into:
k 1 ¼ 2-MV Pchl ð ide Þ
b
ð E455 F660 Þ= 2-MV Pchl ð ide Þ
b
ð E463 F643 Þ
k 2 ¼
2-MV Chl
ð
ide
Þ
b
ð
E455 F660
Þ=
2-MV Chl
ð
ide
Þ
b
ð
E463 F643
Þ
k 3 ¼
2-MV Chl
ð
ide
Þ
b
ð
E463 F643
Þ=
2-MV Chl
ð
ide
Þ
b
ð
E455 F660
Þ
k 4 ¼
2-MV Pchl
ð
ide
Þ
b
ð
E463 F643
Þ=
2-MV Pchl
ð
ide
Þ
b
ð
E455 F660
Þ
Themean of 12 determinations amounted to k 1 ¼
0.0442
0.0052; k 2 ¼
19.7869
1.6540;
k 3 ¼
0.0509
0.0043;
k 4 ¼
22.8827
2.4596;
K 1 ¼
0.9978;
K 2 ¼
0.9978. By substitution of the above constants into Eqs. ( 3.79 )and( 3.80 ), the
latter transform into:
2-MV Pchl
ð
ide
Þ
b
ð
E463 F643
Þ¼
1
:
0022
ð
E463 F643
Þ
0
:
0507
ð
E440 F635
Þ
(3.81)
2-MV Chl
ð
ide
Þ
b
ð
E455 F660
Þ¼
1
:
0022
ð
E455 F660
Þ
0
:
0438
ð
E463 F643
Þ
(3.82)
Search WWH ::




Custom Search