Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 108

Table 3.9 Determination of reliability of equations 3.62 and 3.63 used to calculate excitation amplitudes of MV Pchl9ide) and DV Pchl(ide) at 437 and
451 nm, respectively

Percentage error between
amount of pchlide added
and calcd (%) Mean percentage error n SD
MV Pchlide DV Pchlide DV Pchlide MV Pchlide MV Pchlide DV Pchlide MV Pchlide

Amount of Pchlide added
(pmol/ml)

Amount of Pchlide calcd
(pmol/ml)

Amount of DV MPE added (pmol/ml) DV Pchlide

40 4 36 4 36 0 0 1.24 % 3.7 % 0.32 % 1.9 %

40 8 32 8.7 31.3 8.75 2.2

40 20 20 20 20 0

40 24 16 23.9 16.1 0.41 0.4

40 28 12 28 12 0
40 32 8 31.7 8.3 0.9 3.75
Note : The excitation spectra were recorded at an emission maximum of 625 nm. Other experimental details are as under materials and methods