Biology Reference
In-Depth Information
Table 3.8 Numerical values of constants used in solving 3.21-3.26
Constant determined from excitation
spectra acc. to Eq. (
3.26
)
Mean value of
constant
Constant calcd acc. to
Eqs. (
3.21
) and (
3.22
)
Value of
constant
SD
k
9
0.060
0.006
K
5
0.961
k
10
1.555
0.074
K
6
0.659
k
11
0.530
0.037
K
7
3.326
K
12
0.255
0.002
K
8
0.348
K
13
0.541
0.034
K
9
0.234
K
14
3.510
0.113
K
10
0.962
K
15
0.644
0.029
K
11
0.888
K
16
16.98
1.580
K
12
5.805
K
17
1.896
0.130
K
13
0.260
K
18
0.180
K
19
4.430
0.410
Q
1
0.571
K
20
6.300
0.326
Q
2
0.198
Q
3
0.914
Q
4
0.019
Q
5
0.153
Q
6
0.934
Note: The constants were calculated from fluorescence excitation spectra recorded at 625 samples
of DV MPE, MV Pchlide, and DV Pchlide. Every constant reported in the table is the mean of five
to seven different determinations
0.348
0.022
K
14
Pchlide ratios of the net Soret excitation amplitudes (on the ordinate), was a straight
line that passed through the origin. It exhibited a slope of 0.900 and an index of
correlation of 0.998.
The reliability of Eqs. (
3.60
) and (
3.61
) in determining the proportions of MV
and DV Pchlides in the presence of DV Mpe amounted to 1.24
3.7 % and to
0.32
values for MV and DV Pchlide in the presence and in the absence of various
proportions of DV Mpe.
3.6.5 Calculation of Small Proportions of Monovinyl
Protochlorophyll(ide) in the Presence of Much
Larger Proportions of Divinyl Protochlorophyll(ide)
and in the Presence of Divinyl Mg-Protoporphyrins
To calculate small proportions of MV Pchl(ides) (
<
5 %) in the presence of larger
proportions of DV Pchl(ides) (
95 %) and in the presence of DV Mpe, the Soret
excitation spectrum of the tetrapyrrole mixtures is recorded at an emission wavelength
of 618 nm as was described for Eqs. (
3.57
)and(
3.58
). As a consequence the
(
E
437
F
625), (E451F625), and (E424F625) Soret excitation amplitudes in Eqs. (
3.24
)
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