Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 102

Likewise Eqs. ( 3.52 ) transform into:

k 9 ¼ MV Pchl ð ide Þð E451 F625 Þ= MV Pchl ð ide Þð E437 F625 Þ

k 10 ¼ DV Pchl ð ide Þð E451 F625 Þ= DV Pchl ð ide Þð E437 F625 Þ

k 11 ¼ ð Þ= ð Þ

DV Mpe E451 F625 DV Mpe E437 F625

k 12 ¼ ð Þð Þ= ð Þð Þ

MV Pchl ide E424 F625 MV Pchl ide E437 F625

k 13 ¼ DV Pchl ð ide Þð E424 F625 Þ= DV Pchl ð ide Þð E437 F625 Þ

k 14 ¼ DV Mpe ð E437 F625 Þ= DV Mpe ð E437 F625 Þ

k 15 ¼ DV Pchl ð ide Þð E437 F625 Þ= DV Pchl ð ide Þð E451 F625 Þ

k 16 ¼ MV Pchl ð ide Þð E437 F625 Þ= MV Pchl ð ide Þð E451 F625 Þ

k 17 ¼ DV Mpe ð E437 F625 Þ= DV Mpe ð E451 F625 Þ

k 18 ¼ DV Pchl ð ide Þð E424 F625 Þ= DV Pchl ð ide Þð E451 F625 Þ

k 19 ¼ MV Pchl ð ide Þð E424 F625 Þ= MV Pchl ð ide Þð E451 F625 Þ

k 20 ¼ DV Mpe ð E437 F625 Þ= DV Mpe ð E451 F625 Þ

According to the above terminology, MV Pchl(ides) (E451F625) is as defined
for Eqs. ( 3.55 ). Divinyl MPE (E451F625) refers to the amplitude of the Soret
excitation maximum of authentic DV Mpe at 451 nm, when its Soret excitation
spectrum is recorded at an emission wavelength of 625 nm, etc.. The mean “ k ”
values of five determinations for authentic MV and DV Pchlide and for authentic
DV Mpe are reported in Table 3.8 . Likewise the K 5 to K 14 values as calculated from
Eqs. ( 3.51 ) are also reported in Table 3.8 . The Q 1 to Q 6 values were calculated from
Eqs. ( 3.50 ) and are also reported in Table 3.8 . By substituting for the Q 1 to Q 6
values in Eqs. ( 3.59 ) and ( 3.60 ) and by further reduction of the substituted
equations, the latter transform into:

MV Pchl ð ides Þð E437F625 Þ

¼ 1 : 093 ð E437 F625 Þ 0 : 624 ð E451 F625 Þ 0 : 217 ð E424 F625 Þð 3 : 60a Þ

DV Pchl ð ides Þð E451 F625 Þ

¼ 1 : 070 ð E451 F625 Þ 0 : 020 ð E437 F625 Þ 0 : 163 ð E424 F625 Þð 3 : 61 Þ

The MV Pchl(ides) (E437)/(F625) and DV Pchl(ides) E451 F625 net Soret
excitation ratios, which are calculated from Eqs. ( 3.60 ) and ( 3.61 ), are converted
to authentic ratios of MV to DV Pchl(ides) concentrations by reference to a
standard calibration curve. The latter is constructed exactly as described for
Eqs. ( 3.55 ) and ( 3.56 ). However, in this particular case the various mixtures of
authentic MV Pchlide + DV Pchlide were adjusted to a total of 40 pmol/ml.
Furthermore, in all the tetrapyrrole mixtures, the ratio of DV Mpe + DV Pchlides
was maintained at a molar ratio of 1:1. The curve which related the authentic
MV/DV Pchlide concentration ratios (on the abscissa) to the calculated MV/DV