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determination of the net fluorescence amplitudes at the Soret excitation maxima
of pentacoordinated DV Mg-Proto, and MV Mg-Proto, from two excitation
spectra will be described. One of the two spectra is recorded at an emission
wavelength of 591 nm, in order to optimize the detection of the DV Mg-Proto
Soret excitation maximum at 424-425 nm. The other spectrum is recorded at an
emission wavelength of 587 nm, in order to optimize the detection of the MV
Mg-Proto Soret excitation maximum at 417 nm.
3.5.2.2 Calculation of the Net Fluorescence Amplitudes
at the Monovinyl and Divinyl Soret Excitation Maxima
of Monovinyl and Divinyl Mg-Protoporphyrins in a Mixture
of the Two Tetrapyrroles
Let the 424-nm Soret excitation amplitude of the MV + DV Mg-Proto mixture,
which is recorded at an emission wavelength of 591 nm, be referred to as (E424
F591). Likewise let the 417-nm Soret excitation amplitude of the MV + DV
Mg-Proto mixture, which is recorded at an emission wavelength of 587 nm, be
referred to as (E417 F587). In addition, let X and Y in Eqs. ( 3.37 ) and ( 3.38 )
represent DV Mg-Proto and MV Mg-Proto, respectively. By substituting (E424
F591) for ( E a F b ) and (E417F 587) for ( E c Fd ) in Eqs. ( 3.37 ) and ( 3.38 ), the latters
transform into
DV Mg
Proto
ð
E424 F591
Þ¼½ð
E424 F591
Þð
E417 F587
Þ=
k 2 ð
1
=
K 1 Þ
(3.41)
MV Mg
Proto
ð
E417 F587
Þ¼½ð
E417 F587
Þð
E424 F591
Þ=
k 4
ð
1
=
K 2
Þ
(3.42)
where K 1 and K 2 are as defined by Eq. ( 3.39 )and Where
k 1 ¼
DV Mg Proto
ð
E417F 587
Þ=
DV Mg Proto
ð
E424 F591
Þ
k 2 ¼
MV Mg Proto
ð
E417 F587
Þ=
MVMg Proto
ð
E424 F591
Þ
(3.43)
k 3 ¼
MV Mg Proto
ð
E424 F591
Þ=
MVMg Proto
ð
E417 F587
Þ
k 4 ¼
DV Mg Proto
ð
E424 F591
Þ=
DVMg Proto
ð
E417 F587
Þ
in this context, DV Mg-Proto (E417 F587) refers to the magnitude of the Soret
excitation maximum of authentic DV Mg-Proto at 417 nm, when the Soret excita-
tion spectrum is recorded at an emission wavelength of 587 nm, etc....
The mean k and K values of five determinations for the DV and MV Mg-Proto
pair amounted to: k 1 ¼ 0.130 0.012; k 2 ¼ 9.550 0.450; k 3 ¼ 0.103 0.005;
k 4 ¼
7.750
0.670; K 1 ¼
K 2 ¼
0.987. By substituting for the values of k2, k 4 , K 1
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