Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 89

Table 3.5 Determination of the reliability of Eq. ( 3.36 0 ) which was used in the calculation of the Soret excitation amplitude of Pheo(bide) b at E 438 nm in
mixtures containing Chl a , Chl b , Pheo a and Pheo b

Soret excitation
fluorescence units
at 460 nm Pigments present

Percent error between
amount added and
amount calculated (%)

Mean percent error ( X 4 ) standard

deviation ( Sx 4 ) of the mean per cent error Amount of pigments Chl a Chl b Pheo a Pheo b Added Calculated

Mixture 13: Picomoles per m/ 0 0 0 1940 94.0 93.7 0.3 1.1 % 0.6 %

90.0 89.2 1.0

Percentage of total pigment 0 0 0 100 % 89.1 88.1 1.1

88.8 87.6 1.3

1.9

87.8 86.1

18.8

Mixture 14: Picomoles per m/ 198 195 178 170 8.5 6.9

8.5 7.6 10.6 13.5 % 6.4 %

Percentage of total pigment 27 % 26 % 24 % 23 % 8.5 8.0 5.9

8.5 6.9 18.8

Mixture 15: Picomoles per m/ 220 250 277 120 5.9 6.4 +8.5

5.9 3.6 39.0

Percentage of total pigment 25 % 29 % 32 % 14 % 5.9 4.4 25.4 14.0 % 22.0 %

5.9 5.9 0.0

Mixture 16: Picomoles per m/ 700 750 810 140 6.8 3.1 54.4

6.8 0.1 98.5

Percentage of total pigment 29 % 31 % 34 % 6 % 6.8 0.9 86.7 133.5 % 78.7 %

6.8 9.8 244.1

183.8
The Soret excitation spectra were recorded at an emission maximum of 674 and 660 nm respectively

6.8 5.7