Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 84

Table 3.3 Determination of the reliability of Eq. ( 3.34 0 ) which was used in the calculation of the Soret excitation amplitude of Pheo(bide) a at E 412 nm in
mixtures containing Chl a , Chl b , Pheo a and Pheo b

Soret excitation
fluorescence units at
412 nm

Percent error between
amount added and
amount calculated (%)

Pigments present

Mean percent error ( X 2 ) standard

deviation ( Sx 2 ) of the mean percent error Amount of pigments Chl a Chl b Pheo a Pheo b Added Calculated

Mixture 5: Picomoles per m/ 0 0 275 0 75.9 76.6 +1.0 0.0 % 0.8 %

77.2 76.8 0.5

73.6 74.0 +0.5

Percentage of total pigment 0 0 100 % 0 76.2 75.5 0.9

76.2 76.1 0.1

4.4 % 4.6 %

Mixture 6: Picomoles per m/ 198 195 178 170 54.8 56.4 +2.9

7.1

54.8 50.9

Percentage of total pigment 27 % 26 % 24 % 23 % 54.8 49.9 9.1

54.8 51.9 5.3

54.8 53.0 3.2

Mixture 7: Picomoles per m/ 820 820 314 900 87.6 72.4 17.3 16.8 % 2.6 %

87.6 70.5 19.5

Percentage of total pigment 29 % 29 % 11 % 32 % 87.6 72.6 17.1

87.6 75.9 13.3

Mixture 8: Picomoles per m/ 790 859 116 770 31.3 28.0 10.5 12.9 % 6.7 %

31.3 24.3 22.4

Percentage of total pigment 31 % 34 % 5 % 30 % 31.3 29.2 6.7

12.1
The Soret excitation spectra were recorded at an emission maximum of 674 and 660 nm respectively

31.3 27.5