Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 83

Pheo ð bide Þ a ð F674 E433 Þ¼ ½ A 2 þð A 2 Þð X 2 Þ= 100 ð A 2 Þð S X2 Þ= 100 (3.34)

Where:
A 2 ¼

Eq. ( 3.34 0 )
[(A 2 )( X 2 )/100] ¼ are relative concentration-correction factors, which vanish as
the X values approach zero. The X 2 correction values are listed in Table 3.3 and
their derivation and usage is as for Eq. ( 3.33 ). Finally, the last term in Eqs. ( 3.34 ),
i.e . (A 2 ) (Sx 2 )/100 refers to the standard deviation (Sx 2 ) of the determinations
expressed as a percentage of the pigment content. The Sx 2 values are also listed
in Tables 3.3 and their derivation is as discussed for Eq. ( 3.33 ).
The determination of the X 2 and Sx 2 correction values for various relative
concentrations of Pheo(bide) a are reported in Table 3.3 . The mean per cent error
( X 2 ) and standard deviation (Sx 2 ) ranged from 0.0 + 0.8 % for pure solutions of
Pheo a to 16.8 % 2.6 % for mixtures containing 11 % of Pheo a, and

6.7 % for mixtures containing 5 % of Pheo a.
Based on the results of Table 3.3 , it is therefore recommended that for precise
calculations with Eq. ( 3.2 ), the proper values for X 2 and Sx 2 should be calculated by
interpolation from Table 3.3 . For less rigorous calculations an overall average value
of

12.9 %

8.5 % for X 2 3.7 % for Sx 2 may be utilized.

3.4.3 Calculation of Chloropyll(ide) b

3.4.3.1 Calculation of Chlorophyll and Chlorophyll(ide)
b [Chl(ide)]b in Various Pigment Mixtures

The equation for calculating Chl(ide) b (F660 E460] was derived from the following
simultaneous equations:

ð F660 E4460 Þ¼ Chl ð ide Þ a ð F660 E460 Þþ Pheo ð bide Þ a ð F66604 E460 Þ

þ Chl ð ide Þ b ð F660 E460 Þþ Pheo ð bide Þ b ð F660 E460 Þ

F660 E438 Þ¼þ k 25 Chl ð ide Þ a ð F660 E460 Þþ k 26 Pheo ð bide Þ a ð F660 E460 Þ

þ k 27 Chl ð ide Þ b ð F660 E460 Þþ k 28 Pheo ð bide Þ b ð F660 E460 Þ

F674 E433 Þ¼þ k 29 Chl ð ide Þ a ð F660 E460 Þþ k 30 Pheo ð bide Þ a ð F660 E460 Þ

þ k 31 Chl ð ide Þ b ð F660 E460 Þþ k 32 Pheo ð bide Þ b ð F660 E460 Þ

F674 E412 Þ¼þ k 33 Chl ð ide Þ a ð F660 E60 Þþ k 34 Pheo ð bide Þ a ð F660 E460 Þ

þ k 35 Chl ð ide Þ b ð F660 E460 Þþ k 36 Pheo ð bide Þ b ð F660 E460 Þ