Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 82

F660 E460 Þ¼þ k 17 Chl ð ide Þ a ð F674 E4l2 Þþ k 18 Pheo ð bide Þ a ð F674 E412 Þ

þ k 19 Chl ð ide Þ b ð F674 E412 Þþ k 20 Pheo ð bide Þ b ð F674 E4l2 Þ

F660 E438 Þ¼þ k 21 Chl ð ide Þ a ð F674 E4l2 Þþ k 22 Pheo ð bide Þ a ð F674 E412 Þ

þ k 23 Chl ð ide Þ b ð F674 E412 Þþ k 24 Pheo ð bide Þ b ð F674 E4l2 Þ

Where

k 13 ¼ Chl ð ide Þ a ð F674 E433 Þ= Chl ð ide Þ a ð F674 E412 Þ

k 14 ¼ Pheo ð bide Þ a ð F674 E433 Þ= Pheo ð bide Þ a ð F674 E412 Þ

k 15 ¼ Chl ð ide Þ b ð F674 E433 Þ= Chl ð ide Þ b ð F674 E4I2 Þ

k 16 ¼ Pheo ð bide Þ b ð F674 E433 Þ= Pheo ð bide Þ b ð F674 E412 Þ

k 17 ¼ Chl ð ide Þ a ð F660 E460 Þ= Chl ð ide Þ a ð F674 E412 Þ

k 18 ¼ Pheo ð bide Þ a ð F660 E460 Þ= Pheo ð bide Þ a ð F674 E412 Þ

k 19 ¼ ðÞ b F660 E460 ð Þ= ðÞ b F674 E412 ð Þ

Chl ide Chl ide

k 20 ¼ ð Þ b ð Þ= ð Þ b ð Þ

Pheo bide F660 E460 Pheo bide F674 E412

k 21 ¼ Chl ð ide Þ a ð F660 E438 Þ= Chl ð ide Þ a ð F674 E4I2 Þ

Þ 0 k 22 ¼ ð Þ a ð Þ= ð Þ a ð

Pheo bide F660 E438 Pheo bide F674 E412

k 23 ¼ ð Þ b ð Þ= ð Þ b ð Þ

Chl ide F660 E438 Chl ide F674 E412

k 24 ¼ Pheo ð bide Þ b ð F660 E438 Þ= Pheo ð bide Þ b ð F674 E412 Þ

The numerical values of k 13 - k 24 are reported in Table 3.1 .They were calculated
with the above k equations from the same Soret excitation spectra used to calculate
k 1 - k 12 . Every k valuereportedinTable 3.1 is the mean of 15-25 different
determinations.
By substituting the numerical values of k 13 -k 24 into the four simultaneous
equations described above and by solving for Pheo(bide) a (F674 E412) the
following equation was obtained:

Pheo ð bide Þ a ð F674 E412 Þ¼ 1 : 198 ð F674 E412 Þ 1 : 100 ð F674 E433 Þ

þ 0 : 057 ð F660 E460 Þþ 0 : 110 ð F660 E438 (3.34 0 )

3.4.2.2 Determination of the Reliability of Eq. ( 3.34 0 ) and
Determination of Its X 1 and Sx 1 Correction Factors

The reliability of Eq. ( 3.34 0 ) was tested via the following equation: