Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 80

Table 3.2 Determination of the reliability of Eq. ( 3.33 0 ) which was used in the calculation of the Soret excitation amplitude of Chl a at E 433 nm in mixtures
containing Chl a , Chl b , Pheo a and Pheo b

Soret excitation
fluorescence units
at 433 nm

Percent error between
amount added and
amount calculated

Pigments present

Mean percent error ( X 1 ) standard
deviation ( Sx 1 ) of the mean per cent error Amount of pigments Chl a Chl b Pheo a Pheo b Added Calculated

Mixture 1: Picomoles per m/ 175.0 0.0 0.0 0.0 54.1 54.4 +0.6 % 0.7 % 0.6 %

Percentage of total pigment 100.0 % 0.0 0.0 0.0 53.4 53.3 0.2 %

55.5 56.1 +1.1 %

54.4 55.1 +1.3 %

57.0 57.5 +0.9 %

Mixture 2: Picomoles per m/ 198 195 178 170 61.6 64.3 +4.4 % 2.2 % 2.8 %

Percentage of total pigment 27 % 26 % 24 % 23 % 61.6 63.2 +2.6 %

61.6 63.9 +3.7 %

61.6 59.9 2.8 %

61.6 63.4 +2.9 %

Mixture 3: Picomoles per m/ 90 320 240 280 26.6 28.4 +6.8 % 13.6 % 6.4 %

Percentage of total pigment 10 % 34 % 26 % 30 % 26.6 31.0 +16.5 %

26.6 30.9 +16.2 %

26.6 32.3 +21.4 %

Mixture 4: Picomoles per m/ 170 820 900 900 52.5 60.2 +14.7 % 23.9 % 11.5 %

Percentage of total pigment 6 % 29 % 32 % 32 % 52.5 72.0 +37.1 %
52.5 68.2 +29.9 %
52.5 59.8 +13.9 %
The Soret excitation spectra were recorded at emission maxima of 674 and 660 nm respectively