Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 77

where

k 1 ¼ Chl ð ide Þ a ð F674 E412 Þ= Chl ð ide Þ a ð F674 E433 Þ

k 2 ¼ Pheo ð bide Þ a ð F674 E412 Þ= Pheo ð bide Þ a ð F674 E433 Þ

k 3 ¼ ð Þ b ð Þ= ð Þ b ð Þ

Chl ide F674 E412 Chl ide F674 E433

k 4 ¼ ð Þ b ð Þ= ð Þ b ð Þ

Pheo bide F674 E412 Pheo bide F674 E433

k 5 ¼ Chl ð ide Þ a ð F660 E460 Þ= Chl ð ide Þ a ð F674 E433 Þ

k 6 ¼ Pheo ð bide Þ a ð F660 E460 Þ= Pheo ð bide Þ a ð F674 E433 Þ

k 7 ¼ Chl ð ide Þ b ð F660 E460 Þ= Chl ð ide Þ b ð F674 E433 Þ

k 8 ¼ Pheo ð bide Þ b ð F660 E460 Þ= Pheo ð bide Þ b ð F674 E433 Þ

k 9 ¼ Chl ð ide Þ a ð F660 E438 Þ= Chl ð ide Þ a ð F674 E433 Þ

k 10 ¼ Pheo ð bide Þ a ð F660 E438 Þ= Pheo ð bide Þ a ð F674 E433 Þ

k 11 ¼ Chl ð ide Þ b ð F660 E438 Þ= ChI ð ide Þ b ð F674 E433 Þ

k 12 ¼ Pheo ð bide Þ b ð F660 E438 = Pheo ð bide Þ b ð F674 E433 Þ

The numerical values of k 1 - k l2 are reported in Table 3.1 which is displayed
below.
The k values were calculated with the above k equations, from fluorescence
excitation spectra recorded at 674 and 660 nm, respectively, on purified samples of
Chl a , Chl b , Pheo a and Pheo b . Every k value reported in Table 3.1 is the mean of
15-25 different determinations. By substituting the numerical values of k 1 -k l2 into
the four simultaneous equations described above and by solving for Chl(ide) a
(F674 E433). The following equation was obtained:

Chl ð ide Þ a ð F674 E433 Þ¼ 1 : 248 ð F674 E433 Þ 0 : 145 ð F674 E412 Þ

: ð Þ : ð Þ ð :

33 0 Þ

0 068 F660 E460 0 313 F660 E438 3

3.4.1.2 Determination of the Reliability of Eq. ( 3.33 0 ) and
Determination of Its X 1 and Sx 1 Correction Factors

The reliability of Eq. ( 3.33 0 ) was tested via the following equation:

ð

Chl ð ide Þ a ð F674 E433 Þ¼ A 1 þð A 1 Þð X Þ= 100 A 1 Þð S X1 Þ= 100 (3.33)

Where:
A 1 ¼

Eq. ( 3.33 0 )
[(A 1 )(X ] )/100]

are relative concentration-correction factors, which vanish as
the X values approach zero. The X 1 correction values are listed in Table 3.2 and their
derivation and usage is explained below. Finally, the last term in Eq. ( 3.33 ), i.e . (A 1 )

¼