Biomedical Engineering Reference
In-Depth Information
Fig. 12.6 DNA fingerprinting of the 196 BC2 hybrids using mMaCIR45. Abuab (A) and
Lausigon (L) (abaca check) were run in parallel with the BC2 samples;
Musa balbisiana
(
Mb
)
and “Seniorita” (
S
) were used as positive controls
(B genome). Molecular techniques such as isozymes [
42
-
44
], restriction fragment
length polymorphisms (RFLP) [
36
,
45
], random amplified polymorphic DNA
(RAPD), repetitive elements, diversity of rDNA spacer length (IGS) [
46
],
sequence-tagged site markers (STS), and AFLP [
47
] have been used to study the
origin, relationships, and variability among and within genomic groups in
Musa
.
No studies to date have been done to identify molecular markers that are linked
to disease resistance or to high yield of abaca and bananas. At present, the UPLB's
Institute of Plant Breeding is developing molecular markers to fast-track the
breeding of abaca varieties with high fiber yield and resistance to abaca bunchy
top virus. The genetic background and relationships of different abaca accessions
are being determined by fingerprinting BC
1
and BC
2
populations using a specific
primer set (mMaCIR39, mMaCIR40, and mMaCIR45). This was done to facilitate
the screening for resistance to abaca bunchy top virus and the selection of clones
with high fiber quality (Fig.
12.6
).
Abaca accessions collected from FIDA Bicol, NARC in Leyte, Negros Occi-
dental, and FIDA Davao were also subjected to PCR analysis using the primer set.
Fingerprinting analysis was carried out to establish the genetic relationship among
the different accessions.
Tissue Culture and Genetic Engineering
The basic protocol for abaca in vitro culture has been published. Production of
numerous disease-free abaca varieties through tissue culture has been long realized,
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