Biology Reference
In-Depth Information
Scheme 1
Synthesis of macrocyclic helix-threading peptides
HTPs had a much lower dissociation constant (52 and 271 nM),
both HTPs bound more effectively than a linear counterpart (
9
,
329 nM). Additionally, the macrocyclic HTP was proven capable
in distinguishing between different RNA targets by binding effi -
ciency of approximately two orders of magnitude. HTP affi nity for
a pre-miRNA23b was then examined due to its 5
′
-PyPu-3
′
interca-
lation site fl anked by 3
M, indicating
the applicability of these HTPs to miRNA maturation. Optimally,
this binding interaction should function in a comparable fashion to
′
bulges, and found to be 2.3
μ
