Biology Reference
In-Depth Information
of great interest is the study published by Farace et al.
identifying p27
Kip1
mRNA as a possible target of miR-221
[
19
]. This fi nding is very intriguing, since p27
Kip1
has been
proposed as a tumor-suppressor gene, which is down-regulated
in several types of tumors. These data support the concept that
targeting miR-221 with antagomiR molecules might lead to
an increased expression of the tumor-suppressor p27
Kip1
, bring-
ing novel treatment options in cancer treatment.
Acknowledgments
This work was partially supported by a grant from MIUR (PRIN09
grant n. 20093N774P “Molecular recognition of microRNA
(miR) by modifi ed PNA: from structure to activity”). R.G. is
granted by Fondazione Cariparo (Cassa di Risparmio di Padova e
Rovigo), by UE THALAMOSS (THALAssaemia MOdular
Stratifi cation System for personalized therapy of beta-thalassemia),
by Telethon (contract GGP10214) and AIRC (Italian Association
for Cancer Research). This research is also supported by CIB
(Interuniversity Consortium for Biotechnologies).
References
1. He L, Hannon GJ (2010) MicroRNAs: small
RNAs with a big role in gene regulation. Nat
Rev Genet 5:522-531
2. Manicardi A, Fabbri E, Tedeschi T, Sforza S,
Bianchi N et al (2012) Cellular Uptakes,
Biostabilities and Anti-miR-210 Activities of
Chiral Arginine-PNAs in Leukaemic K562
Cells. Chembiochem 13:1327-1337
3. Fabbri E, Manicardi A, Tedeschi T, Sforza S,
Bianchi N et al (2011) Modulation of the bio-
logical activity of microRNA-210 with peptide
nucleic acids (PNAs). ChemMedChem 6:
2192-2202
4. Fabbri E, Brognara E, Borgatti M, Lampronti
I, Finotti A et al (2011) miRNA therapeutics:
delivery and biological activity of peptide
nucleic acids targeting miRNAs. Epigenomics
3:733-745
5. Gambari R, Fabbri E, Borgatti M, Lampronti I,
Finotti A et al (2011) Targeting microRNAs
involved in human diseases: a novel approach for
modifi cation of gene expression and drug devel-
opment. Biochem Pharmacol 82:1416-1429
6. Nielsen PE, Egholm M, Berg RH, Buchardt O
(1991) Sequence-selective recognition of DNA
by strand displacement with a thymine-
substituted polyamide. Science 254:1497-1500
7. Nastruzzi C, Cortesi R, Esposito E, Gambari R,
Borgatti M et al (2000) Liposomes as carriers
for DNA-PNA hybrids. J Control Release
68:237-249
8. Karkare S, Bhatnagar D (2006) Promising
nucleic acid analogs and mimics: characteristic
features and applications of PNA, LNA, and
morpholino. Appl Microbiol Biotechnol 71:
575-586
9. Tonelli R, Fronza S, Purgato S, Camerin C,
Bologna F et al (2005) Anti-gene Peptide
Nucleic Acid (PNA) Specifi cally Inhibits
N-myc Expression in Human Neuroblastoma
Cells Leading to Persistent Cell-growth
Inhibition and Apoptosis. Mol Cancer Ther
4:779-786
10. Nielsen PE (2001) Targeting double stranded
DNA with peptide nucleic acid (PNA). Curr
Med Chem 8:545-550
11. Borgatti M, Lampronti I, Romanelli A, Pedone C,
Saviano M et al (2003) Transcription factor
decoy molecules based on a peptide nucleic
acid (PNA)-DNA chimera mimicking Sp1
binding sites. J Biol Chem 278:7500-7509
12. Rasmussen FW, Bendifallah N, Zachar V,
Shiraishi T, Fink T et al (2006) Evaluation of
transfection protocols for unmodifi ed and
modifi ed peptide nucleic acid (PNA) oligo-
mers. Oligonucleotides 16:43-57
13. Cortesi R, Mischiati C, Borgatti M, Breda L,
Romanelli A et al (2004) Formulations for
