Biology Reference
In-Depth Information
Fig.
3
(
a, b
) Quantifi cation by real-time PCR of miRNA-210 in Mithramycin-induced K562 cells. Effects of
treatment with PNA-TAT and NLS-PNA-TAT, 1
M (
b
) on miRNA-210 content in cells cultured for
48 h in the presence of MTH 15nM. (
c
) Effects on miR-210 and pri-miR-210 after 72 h treatment of K562 cells
with 2
μ
M (
a
) and 2
μ
M PNA-TAT-mis1, PNA-TATmis2 (control sequences
containing 1 mismatch as compared to PNA-TAT), and PNA-TAT on pri-miR-210. The data represent the aver-
age ± SD (
n
= 3) (*
p
< 0.05) (reproduced from ref. [
9
] )
μ
M PNA-TAT. (
d
) Effects of 24 h treatment with 1
μ
4
Notes
1. Carry out all synthetic protocols under the fume hood, wear-
ing appropriate protections (gloves and glasses).
2. Always prepare fresh solutions for activators, capping and
deblock.
3. Protect the capping and deblock solutions from the light.
4. Steps from Subheading
3.3
,
step 8
to Subheading
3.3
,
step 11
and all work with FITC-modifi ed oligomers need to be always
carried out in the dark.
