Biology Reference
In-Depth Information
and another to the BRCA system for pre-miRNA B. Again, be
sure to include all controls.
12. If the slope of the BRCA seems too low, you have the chance
of calibration, either with mature miRNA or with a DNA
primer corresponding to the 5
-segment of the pre-miRNA.
The latter test is also highly recommended for testing the
BRCA independent of miRNA maturation during
troubleshooting.
′
Acknowledgement
The authors would like to thank the Deutsche Forschungsge-
meinschaft (DFG) for ongoing generous support.
References
1. Croce CM (2011) miRNAs in the spotlight:
understanding cancer gene dependency. Nat
Med 17:935-936
2. Pfeifer A, Lehmann H (2010) Pharmacological
potential of RNAi-focus on miRNA. Pharmacol
Therapeut 126:217-227
3. Medina PP, Slack FJ (2008) microRNAs and
cancer: an overview. Cell Cycle 7:2485-2492
4. Arenz C (2006) MicroRNAs-future drug tar-
gets? Angew Chem Int Ed Engl 45:
5048-5050
5. Neubacher S, Dojahn CM, Arenz C (2011) A
rapid assay for miRNA maturation by using
unmodifi ed
cellular function for the RNA-interference
enzyme Dicer in the maturation of the let-7
small temporal RNA. Science 293:834-838
12. miScript II RT Kit, Cat. # 218161.
13. DiNitto JP, Wang LY, Wu JC (2010)
Continuous fl uorescence-based method for
assessing Dicer cleavage effi ciency reveals 3 '
overhang nucleotide preference. Biotechniques
48:303-309
14. Davies BP, Arenz C (2006) A homogenous
assay for micro RNA maturation. Angew Chem
Int Ed Engl 45:5550-5552
15. Davies BP, Arenz C (2008) A fl uorescence
probe for assaying micro RNA maturation.
Bioorg Med Chem 16:49-55
16. Gumireddy K, Young DD, Xiong X, Hogenesch
JB, Huang Q, Deiters A (2008) Small-molecule
inhibitors of microrna miR-21 function. Angew
Chem Int Ed Engl 47:7482-7484
17. Young DD, Connelly CM, Grohmann C,
Deiters A (2010) Small molecule modifi ers of
microRNA miR-122 function for the treatment
of hepatitis C virus infection and hepatocellular
carcinoma. J Am Chem Soc 132:7976-7981
18. Bose D, Jayaraj G, Suryawanshi H, Agarwala P,
Pore SK, Banerjee R, Maiti S (2012) The
tuberculosis drug streptomycin as a potential
cancer therapeutic: inhibition of miR-21 func-
tion by directly targeting its precursor. Angew
Chem Int Ed 51:1019-1023
19. Jonstrup SP, Koch J, Kjems J (2006) A
microRNA detection system based on padlock
probes and rolling circle amplifi cation. RNA
12:1747-1752
20. Cheng Y, Zhang X, Li Z, Jiao X, Wang Y,
Zhang Y (2009) Highly sensitive determina-
tion of microRNA using target-primed and
branched rolling-circle amplifi cation. Angew
Chem Int Ed 48:3268-3272
pre-miRNA.
Chembiochem
12:2302-2305
6. Takeshita D, Zenno S, Lee WC, Nagata K,
Saigo K, Tanokura M (2007) Homodimeric
structure and double-stranded RNA cleavage
activity of the C-terminal RNase III domain of
human dicer. J Mol Biol 374:106-120
7. MacRae IJ, Doudna JA (2007) Ribonuclease
revisited: structural insights into ribonuclease
III family enzymes. Curr Opin Struct Biol
17:138-145
8. Hellani A, Schuchman EH, Al-Odaib A, Al
Aqueel A, Jaroudi K, Ozand P, Coskun S
(2004) Preimplantation genetic diagnosis for
Niemann-Pick disease type B. Prenat Diagn
24:943-948
9. Zhang H, Kolb FA, Jaskiewicz L, Westhof E,
Filipowicz W (2004) Single processing center
models for human Dicer and bacterial RNase
III. Cell 118:57-68
10. Starega-Roslan J, Koscianska E, Kozlowski P,
Krzyzosiak WJ (2011) The role of the precur-
sor structure in the biogenesis of microRNA.
Cell Mol Life Sci 68:2859-2871
11. Hutvagner G, McLachlan J, Pasquinelli AE,
Balint E, Tuschl T, Zamore PD (2001) A
