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7. Investigating two different components by FCS, both have to
differ significantly regarding diffusion time or molar mass,
respectively.
8. Replacing divalent cations leads to inhibition of Dicer activity.
9. Dicer exclusively cleaves dsRNA and not ssRNA. Nevertheless,
diffusion time can increase slightly after Dicer addition due to
Dicer affinity to 5′-terminal modifications of ssRNA [
12
].
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