Biomedical Engineering Reference
In-Depth Information
HA
(-ve charge)
PEI
(+ve charge)
+
Cyanine 3 (Cy3)-labelled
siRNA (-ve charge)
PEI modified HA
Size of nanoparticle:
~50 to 80 nm (by dynamic light
scattering analysis)
Surface charge of nanoparticle: -6 to -15mV
zeta potential measurements(by)
HA-PEI/siRNA (nanoparticle)
Figure 3.3
Schematic representation of HA-PEI/siRNA nanosystem
suggesting a positive core containing the siRNA with a negatively-
charged HA shell structure. Adapted from S. Ganesh, A.K. Iyer,
D.V. Morrissey and M.M. Amiji,
Biomaterials
, 2013,
34
, 3489
[19]
3.3.3 Demonstrating Receptor-specific Uptake using
Hyaluronic Acid-based Particles and Small Interfering
Ribonucleic Acid
With their cholanic acid conjugated HA derivatives, Choi and
co-workers, demonstrated specific cell uptake in CD44 expressing
cells [7]. When they treated the CD44-over expressing squamous
cell carcinoma (SCC7) cells with fluorescently-labelled Cyanine 5.5
(Cy5.5) NP, they detected strong signals in the cytosol suggesting
efficient intracellular uptake by a receptor-mediated pathway. In
addition, when they pretreated the cells with a higher dose of free
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