Biomedical Engineering Reference
In-Depth Information
Positive feedback in cellular differentiation has been demonstrated
by many studies. In a report, HA conjugated with catechol exhibited
adhesiveness as the material surface for NSC culture, when used
as 3D hydrogels to encapsulate the NSC, and they also show high
compatibility [48]. The improvement of NSC attachment on the
scaffold is also achieved by modification with NgR antibody or
PLL, and then the viability and differentiation of NSC on the HA
hydrogel are raised, as shown in the study of Pan and co-workers
[49]. Similarly, Hou and co-workers found that cell infiltration
and neurite extension are promoted by HA hydrogels modified
with laminin in a cortical lesion [21]. Also, if the HA hydrogel is
modified by dextran, which acts as a substrate for stem cell adhesion,
the stem cell viability and spreading are enhanced, as reported
by Ananthanarayanan and co-workers [47]. Meanwhile, Jha and
co-workers [46] have confirmed that immobilisation of gelatin on
HA hydrogel may provide cell-adhesion modules and play a major
part in directing the differentiation of mesenchymal stem cells (MSC).
(a)
5 µm
Figure 1.2
SEM image showing neural cells adhered to the HA-
poly-D-lysine hydrogel. Reproduced with permission from
W.M. Tian, S.P. Hou, J. Ma, C.L. Zhang, Q.Y. Xu, I.S. Lee,
H.D. Li, M. Spector and F.Z. Cui,
Tissue Engineering
, 2005,
11
,
3-4, 513. ©2005, Mary Ann Liebert Inc [20]
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