Inhibition of Free Radical Degradation in Medical Grade Hyaluronic Acid - Hyaluronic Acid for Biomedical and Pharmaceutical Applications - page 114

pH 4) the H · radical donating property of GSH is less effective (cf.
Figures 5.4 and 5.5 ). According to the observations presented in
Figure 5.3 this diminished protection by GSH originated from the
acetic acid itself. Thus, during the HA extraction procedure, one
should pay attention both to the pH value applied and the types of
acid. In any case, however, the application of GSH to protect high
MW hyaluronan against its free radical degradation is proved by
the above experiments.

Recently, according to the paper by Sunitha and coworkers [31], an
excellent inhibitory action of GSH against hyaluronidase enzymes
was demonstrated. Thus, applying GSH during the isolation of high
MW HA by extraction procedures from tissues contaminated by
hyaluronidases or other media containing trace amounts of transition
metals is an important new step.

Acknowledgment

This work was supported by the grant VEGA 2/0011/11 of the
Scientific Grant Agency of the Ministry of Education, Science,
Research and Sport of the Slovak Republic and Slovak Academy of
Science. We express thanks also to the Slovak Academic Information
Agency (SAIA) for providing a postdoctoral scholarship for
Dr. Tamer, and to our director Dr. M. Dubovický for accepting him
at our institute.

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