TOXICITY OF CARBON NANOTUBES - Carbon Nanotubes: From Bench Chemistry to Promising Biomedical Applications - page 227

17 Inluence of length

on cytotoxicity
of multi-walled
carbon nanotubes
against human
acute monocytic
leukaemia cell line
THP-1 in vitro and
subcutaneous tissue
of rats in vivo

Molecular
Biosystems ,
2005, 1 , 176-
182

Puriied,
acid-treated
MWCNTs of
two different
lengths:
1.

Human acute
monocytic
leukaemia cell line
(THP-1)

Cytotoxic effects was based
on the production of TNF-α
Both 220-CNTs and
825-CNTs possess
induction activity towards
macrophages.

Concentration range
used: 5-50 ng/mL

220 nm

2. 825 nm

18 Multi-walled

carbon nanotube
interactions with
human epidermal
keratinocytes

Toxicology
Letters , 2005,
155 (3), 377-
384

Self-prepared
MWCNTs

Human Epidermal
Keratinocytes
(HEK)

HEK viability assessed by the
NR assay slightly decreased
in a dose-dependent manner
at 24 and 48 hours (data not
shown) after exposure to the
nanotubes

Concentration range
used: 0.1, 0.2, and
0.4 mg/mL

19 Carbon nanotube

biocompatibility
with cardiac muscle
cells

Institute
of Physics
Publishing
Nanotechnology ,
2006, 17 ,
391-397

Highly puriied
SWCNTs.

Cardiac muscle
cell lines (rat cell
line H9c2)

SWCNTs can be seeded with
cardiomyocytes without
affecting cell viability

Concentration range
used:
0.2 mg/mL

20 In-vitro toxicity

assessment of single
and multi-walled
carbon nanotubes in
human astrocytoma
and lung carcinoma
cells

Toxicology
Letters , 2007,
172S , S1-S240

SWCNTs 1. MWCNTs

2. MWCNT-
COOH
3. MWCNT-
NH 2

1. Human

astrocytoma
D384-cells

MTT results revealed a
strong cytotoxicity
Calcein/PI staining did not
conirm MTT cytotoxicity in
both cell lines

Concentration range
used:
0.1-100 μg/mL

2. Lung

carcinoma
A549-cells