Biomedical Engineering Reference
In-Depth Information
Abarrategi et al. 106 combined the idea of polymer-CNT composite and CNT-
BMP complex and studied the co-effect of CNT/chitosan/rhBMP-2. The growth
of bone cells C2C12 was examined after histological stains. Observation of
the cross-section of the microchannelled structure of CNT/chitosan showed
that cells could penetrate into the scaffold cavities and attach on its walls,
suggesting that cellular iniltration into the porous structure was possible
(Fig. 7.11a). The depth of the cell penetration might be limited by nutrient
diffusion. Furthermore, rhBMP-2 greatly enhanced the formation of bone.
Alkaline phosphatase (ALP) activity increased by many times in the presence
of rhBMP-2 (Fig. 7.11b).
(a)
(b)
Figure 7.11 (a) Microscope images of SMA immunohistochemical stained C2C12
cells penetrating into the scaffold structure. (b) rhBMP-2 signiicantly enhanced
the proliferation of C2C12 cells in the scaffold. Reproduced from Gabay et al. 111 with
permission.
Wang et al. 107 proved the in vivo activity of CNT-based materials for bone
cell proliferation. The CNT-polycarbosilane composite implanted under
subcutaneous tissue were covered by surrounding ibrous connective tissue
after 1 week. Some inlammation appeared but did not worsen after 4 weeks.
Those composite implants in hard tissue simulated callus formation. After 4
weeks some parts of the newly formed bone attached to the composite.
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