Biomedical Engineering Reference
In-Depth Information
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B
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C
Figure 4.7
Binding of different concentrations (in mU) of methionine-7-amido-4-methylcoumarin (MET-AMC)
in solution in the cSPA (competitive scintillation proximity aminoacyl-tRNA synthetase charging
assay)
(
Forbes et al., 2007
): (a) 0.05 (b) 0.025 (c) 0.01.
of the binding rate coefficient,
k
, and the fractal dimension,
D
f
, for a single-fractal analysis
are given in
Table 4.6
.
Figure 4.7b
shows the binding of 0.025 mU of MET-AMC in solution in the cSPA (
Forbes
et al., 2007
). A single-fractal analysis is adequate to describe the binding kinetics. The values
of the binding rate coefficient,
k
, and the fractal dimension,
D
f
, for a single-fractal analysis
are given in
Table 4.6
. A decrease in the MET-AMC concentration in solution by a factor
of 2 from a value of 0.05 to 0.025 mU leads to a decrease in the binding rate coefficient
by a factor of 2.55 from a value of 66.248 to 26.008.
Figure 4.7c
shows the binding of 0.01 mU of MET-AMC in solution in the cSPA (
Forbes
et al., 2007
). A single-fractal analysis is adequate to describe the binding kinetics. The values
of the binding rate coefficient,
k
, and the fractal dimension,
D
f
, for a single-fractal analysis
are given in
Table 4.6
. In this case, a decrease in the MET-AMC concentration in solution
by factor of 2.5 from a value of 0.025 to 0.01 mU in solution leads to a decrease in the bind-
ing rate coefficient,
k
, by a factor of 2.44 from a value of 26.008 to 10.644.