Biomedical Engineering Reference
In-Depth Information
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Figure 4.7
Binding of different concentrations (in mU) of methionine-7-amido-4-methylcoumarin (MET-AMC)
in solution in the cSPA (competitive scintillation proximity aminoacyl-tRNA synthetase charging
assay) ( Forbes et al., 2007 ): (a) 0.05 (b) 0.025 (c) 0.01.
of the binding rate coefficient, k , and the fractal dimension, D f , for a single-fractal analysis
are given in Table 4.6 .
Figure 4.7b shows the binding of 0.025 mU of MET-AMC in solution in the cSPA ( Forbes
et al., 2007 ). A single-fractal analysis is adequate to describe the binding kinetics. The values
of the binding rate coefficient, k , and the fractal dimension, D f , for a single-fractal analysis
are given in Table 4.6 . A decrease in the MET-AMC concentration in solution by a factor
of 2 from a value of 0.05 to 0.025 mU leads to a decrease in the binding rate coefficient
by a factor of 2.55 from a value of 66.248 to 26.008.
Figure 4.7c shows the binding of 0.01 mU of MET-AMC in solution in the cSPA ( Forbes
et al., 2007 ). A single-fractal analysis is adequate to describe the binding kinetics. The values
of the binding rate coefficient, k , and the fractal dimension, D f , for a single-fractal analysis
are given in Table 4.6 . In this case, a decrease in the MET-AMC concentration in solution
by factor of 2.5 from a value of 0.025 to 0.01 mU in solution leads to a decrease in the bind-
ing rate coefficient, k , by a factor of 2.44 from a value of 26.008 to 10.644.
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