Biomedical Engineering Reference
In-Depth Information
Table 16.10: Binding rate coefficients for 50 nM complementary oligonucleotide and one base mismatch to the molecular
beacon (MB) (
Li et al., 2001
).
Target in Solution/Molecular Beacon (MB) on
Surface
Immobilization
Method
k
k
1
k
2
10
5
10
5
50 nM 5
0
-GCG ACC ATA GCG ATT TAG(A-3
0
)/
MB (5
0
-TMR-CCT AGC TCT AAA TCG CTA TGG
TCG CGC (biotin dT)AG G-DABCYL-3
0
)
BSA-streptavidin-biotin 0.006209
0.003251
5
1.8
20.1984
0.0137
50 nM 5
0
-GCG ACC ATA GCG ATT TAG(A-3
0
)/
MB (5
0
-TMR-CCT AGC TCT AAA TCG CTA
TGG TCG CGC (biotin dT)AG G-DABCYL-3
0
)
(one base mismatch)
BSA-streptavidin-biotin
0.00136
0.00059
0.000117
0.000042 0.08915
0.00328
Table 16.11: Fractal dimensions for 50 nM complementary oligonucleotide and one base mismatch to the molecular beacon (MB)
(
Li et al., 2001
).
Complementary Target in Solution/Molecular Beacon (MB)
on Surface
Immobilization
Method
D
f
D
f1
D
f2
50 nM 5
0
-GCG ACC ATA GCG ATT TAG(A-3
0
)/MB
(5
0
-TMR-CCT AGC TCT AAA TCG CTA TGG TCG CGC
(biotin dT)AG G-DABCYL-3
0
)
BSA-Streptavidin-biotin 1.4492
0.2452
0
þ
0.6076 2.4386
0.0816
50 nM 5
0
-GCG ACC ATA GCG ATT TAG(A-3
0
)/MB
(5
0
-TMR-CCT AGC TCT AAA TCG CTA TGG TCG CGC
(biotin dT)AG G-DABCYL-3
0
) (one base mismatch)
BSA-streptavidin-biotin 1.2264 0.1986 0.268 þ 0.3290 2.4330 0.0652
