Biomedical Engineering Reference
In-Depth Information
Table 16.6: Binding (corrected) of 500 pM cy3-labelled targets to Area 1, Area 2, and Area 3 on a 20-mer capture probe spotted
on glass (
Schultz et al., 2008
).
Area Number
k
k
1
k
2
D
f
D
f1
D
f2
One
0.5946 0.1037
0.2108 0.0207
7.908 0.100
1.5288 0.0998
1.1008 0.1100
2.3186 0.03638
Two (control)
0.3441 0.0644
0.1378 0.0145
5.680 0.122
1.5068 0.1066
1.1342 0.1018
2.3628 0.0614
Three
0.5751
0.0990
0.1923
0.0213
5.684
0.060
1.6018
0.0940
1.1520
0.1244
2.2946
0.02624
Influence of the vignetting effect (distance of area from the detector).
