Biomedical Engineering Reference
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Table 16.2: Binding of 40 nM FAM-SP-2, 5.6 nM/s T4PNK, and 1 mM ATP to the singly labeled DNA-hairpin probe coupled
with
l
exonuclease at pH 8.0.
l
exonuclease
k
k 1
k 2
D f
D f1
D f2
0.5
69.96
65.16
58.33
6.93
110.31
7.19
0.906
0.338
0.822
0.114
1.213
0.1578
1.0
118.57
34.29
50.75
9.98
1109.57
29.72
1.0532
0.1124
0.458
0.143
2.004
0.0464
2.5
241.15
87.74
98.96
23.1
4750.65
133.47
13224
0.1658
0.690
0.189
2.638
0.0682
Influence of
l
exonuclease ( Song and Zhao, 2009 ).
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