Binding and Dissociation Kinetics During Hybridization on Biosensor Surfaces - Biosensors and Biosensor Kinetics - page 450

Table 16.1: Binding rate coefficients and fractal dimensions for the binding of FAM-SP-2 þ lambda exo þ T4PNK and

FAB-SP-2p þ lambda exo in solution to the G-quenched singly-labeled DNA-hairpin smart probe coupled with l exonuclease

( Song and Zhao, 2009 ).

Analyte in Solution/
Receptor on Surface k k 1 k 2 D f D f1 D f2

40 nM FAM þ

lambda exo (10 units)/G
quenched smart probe

þ SP2 1972.38 512.55 640.96 124.0 5343.91 63.44 2.5754 0.1088 1.9390 0.2090 2.9296 0.02314

40 nM FAM þ SP2 þ
lambda exo (10 units)

4.576 0.439 na na 0.9110 0.0986 na na

þ

40 nM 4PNK//G
quenched smart
probe