Biomedical Engineering Reference
In-Depth Information
CHAPTER 16
Binding and Dissociation Kinetics During
Hybridization on Biosensor Surfaces
Chapter Outline
16.1 Introduction
449
16.2 Theory
450
16.2.1 Single-Fractal Analysis
451
Binding Rate Coefficient
451
Dissociation Rate Coefficient
451
16.2.2 Dual-Fractal Analysis
452
Binding Rate Coefficient
452
16.1 Introduction
Mao et al. (2009)
recently reported that the nucleic acid test is important in the diagnosis and
treatment of genetic disorders, for the detection of infectious agents, drug discovery, and in
the warnings against biowarfare agents (
Wang, 1999; Palacek and Fojta, 2001; Gooding,
2002; Drummond et al., 2003
). Although new methods such as real-time polymerase chain
reaction (RT-PCR) (
Kaltenbeck and Wang, 2005
), DNA microarrays (gene chip) (
Brown
and Botstein, 1999
), SPR BIAcore instrument (
http://wwwbiacore.com
)
, and GeneXpert sys-
tem (
Petersen et al., 1999
) are fast and sensitive tools to detect nucleic acid sequences, there
is a high equipment cost and a need for highly-trained personnel. An ideal tool is required for
fast, sensitive, low-cost, and easy-to-use detection of nucleic acids (
Piunno and Krull, 2005;
Hahns et al., 2005
).
Mao et al. (2009)
point out that nucleic acid biosensors may be good can-
didates to meet these standards.
Odenthal and Gooding (2007) and Leung et al. (2007)
have
reported on the use of different nucleic acid biosensors with different transducers.
Nanomaterial labels and novel signal amplification strategies labels have increased sensitiv-
ity (
Wang, 2005
).
Mao et al. (2009)
have recently reported on disposable nucleic acid
biosensors based on gold nanoparticle probes and a lateral flow strip.
