Biomedical Engineering Reference
In-Depth Information
Table 15.5: Binding rate coefficients and fractal dimensions for transcription factors to microcantilever arrays
(
Huber et al., 2006
).
Analyte in
Solution/Receptor
on Surface
k
k
1
k
2
D
f
D
f1
D
f2
80 nM rhSPl/
rhNF-
22.750
3.380 12.367
1.941
49.233
0.322
2.82412
1.2598
1.3908
0.2476
2.2030
0.01719
B binding
oligonucleotide
k
100 nM rhNF-
k
B/
SP1-binding
oligonucleotide
16.941 1.773 13.708 1.620
33.166 0.326
2.4926 0.07238 2.2742 0.1797
2.88748 0.02348
200 nM rhSPlin SP1
binding buffer
18.843 0.621 na
na
2.3718 0.4756
na
na
400 nM rhNF-
k
B
5.786
0.115 na
na
1.6270
0.01876 na
na
in NF-
k
B binding
buffer
