Biomedical Engineering Reference
In-Depth Information
700
1000
600
500
400
300
800
600
400
200
100
200
0
0
0
0
20
40
60
80
100
120
140
20
40
60 80
Time (s)
100
120
140
A
B
Time (s)
500
400
300
200
100
0
0
20
40
60
80
100
120
140
C
Time (s)
Figure 14.10
Binding of different concentrations of STX (in ng/mL) in solution premixed with GT13A-antibody to
the STX immobilized on a chip surface in a Biacore Q SPR biosensor (
Fonfria et al., 2007
): (a) 0,
(b) 5, (c) 10. When only a solid line (--) is used then a single-fractal analysis applies. When both
a dashed (- - -) and a solid (--) line are used then the dashed line represents a single-fractal
analysis and the solid line represents a dual-fractal analysis, and the dashed line represents the
single-fractal analysis. In both cases, the solid line is the best fit line.
Table 14.8: Binding rate coefficients and fractal dimensions for paralytic shellfish poisoning
(PSP) toxins in solution to surface plasmon resonance (SPR)-based biosensor in shellfish
matrices (
Fonfria et al., 2007
).
STX
Concentration
(ng/mL)
k
k
1
k
2
D
f
D
f1
D
f2
0
20.626
6.087
9.473 0.642
600 0.0 1.4850 0.1835 0.9576
0.0755
3.0 7.5E-14
5
16.563
4.489
8.183 0.253
450 0.0 1.520 0.170
1.0446
0.0351
3.0 8.4E-14
10
11.336
5.601
0.224
325
0.0 1.4966
0.1718 1.1222
3.0
9.0E-14
0.103
0.0450
An anti-GTX/3 antibody immobilized on a saxitoxin (STX) CM5 chip.
