Biomedical Engineering Reference
In-Depth Information
Table 14.6: Binding and dissociation rate coefficients for different concentrations of Staphylococcal enterotoxin B (SEB) in solution
to monoclonal antibody-functionalized microbeads on a sensor chip (
Haes et al., 2006
).
Analyte in Solution/
Receptor on Surface
k
k
1
k
2
k
d
k
d1
k
d2
1 nM SEB/monoclonal
antibody-functionalized
microbeads on sensor chip
0.0130
0.00698
0.000827
0.000250
0.4156
0.5201
0.2078
0.2309
0.0347
3.6949
0.023
0.0341
10 pM SEB/monoclonal
antibody-functionalized
microbeads on sensor chip
0.002975
0.000725 na
na
0.1213
0.0286
na
na
100 fM SEB/monoclonal
antibody-functionalized
microbeads on sensor chip
0.00638 0.000314
0.01438
0.00198
0.02969
0.00198
0.02997 0.01465
0.009114 0.000876 0.4782 0.0135
10 fM SEB/monoclonal
antibody-functionalized
microbeads on sensor chip
0.002653 0.000655 na
na
0.02650 0.00933
0.01598 0.00524
0.4622 0.002
1 fM SEB/monoclonal
antibody-functionalized
microbeads on sensor chip
0.000416
0.00015
na
na
0.008844
0.00577 0.001526
0.000755 0.526
0.0037
