Biomedical Engineering Reference
In-Depth Information
300
300
250
250
200
200
150
150
100
100
50
50
0
0
0
50
100
150
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350
0
50
100
150 200
Time (s)
250
300
350
A
B
Time (s)
120
250
100
200
80
150
60
100
40
50
20
0
0
0
50
100
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350
0
50
100
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350
C
D
Figure 13.6
Binding of different units of restriction endonuclease activity using a molecular beacon
( Ma et al., 2007 ): (a) 32, (b) 25, (c) 10, (d) 4.
Time (s)
Time (s)
Figure 13.6d shows the real-time monitoring of the DNA cleavage process catalyzed by 14
units of Rsa I endonuclease. Once again, a single-fractal analysis is adequate to describe the
binding reaction. The values of the binding rate coefficient k and the fractal dimension D f
for a single-fractal analysis are given in Table 13.5 .
Figure 13.7a and Table 13.5 show the increase in the binding rate coefficient k with an
increase in the restriction endonuclease concentration in units, U. For the data shown in
Figure 13.7a , the binding rate coefficient k is given by:
1
:
593
0
:
349
k
¼ð
0
:
0268
0
:
0209
Þ½
endonuclease, unit
ð
13
:
5a
Þ
The fit is good. Only three data points are available. The availability of more data points
would lead to a more reliable fit. The binding rate coefficient k is sensitive to the
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