Biomedical Engineering Reference
In-Depth Information
Table 13.3: Binding and dissociation rate coefficients for TNT (trinitrotoluene) in aqueous
environment to anti-TNT mAb (anti-nitorphenol monoclonal antibody, mAb) by a highly sensitive
TNT immunosensor with a prototype fluorescence-based detection system (KinexA Inline
Biosensor, Sappidyne Insrtrument, Inc.;
Bromage et al., 2007
).
TNT concentration
(
g/L)
k
k
d
k
d1
k
d2
m
0
0.0239
0.0037
0.03336
0.00205
na
na
0.01
0.0206
0.002
0.03347
0.00202
na
na
0.05
0.02248
þ
0.0337
0.0594
0.0075
na
na
0.5
0.01935
0.00027 0.08749
0.0027
na
na
5
0.03409
0.00547
0.0119
0.0216
0.00546
þ
0.0244
0.1471
0.00042
25
0.02962 0.00375 0.02437 þ 0.02677 4.3E-05 þ 5.7E-05 0.1843 0.0016
Table 13.4: Fractal dimensions for the binding and the dissociation phase for TNT
(trinitrotoluene) in aqueous environment to anti-TNT mAb (anti-nitorphenol monoclonal
antibody, mAb) by a highly sensitive TNT immunosensor with a prototype fluorescence-based
detection system (KinexA Inline Biosensor, Sappidyne Insrtrument, Inc.;
Bromage et al., 2007
).
TNT concentration (
m
g/L)
D
f
D
fd
D
fd1
D
fd2
0
0.812
0.176
2.2506
0.0340
na
na
0.01
0.6966
0.1920
2.2582
0.03302
na
na
0.05
0.7816
þ
0.1727
2.2662
0.1073
na
na
0.5
0.966
0.0180
2.6742
0.0017
na
na
5
1.6718
0.2162
1.676
0.560
0.594
þ
1.864 2.9172
0.00866
25
1.6162 0.2184
2.0098 0.5368
0 þ 0
2.9740 0.0130
single-fractal analysis, and (b) the dissociation rate coefficient
k
d
and the fractal dimension for
dissociation
D
fd
are given in
Tables 13.3
and
13.4
.
Figure 13.3d
shows the binding and dissociation of 0.5
m
g/L of TNT in the aqueous environment
to anti-TNT mAb by a highly sensitive TNT immunosensor with a prototype fluorescence-based
detection system (KinExA Inline Biosensor, Sappidyne Instrument, Inc.;
Bromage et al., 2007
).
Once again, a single-fractal analysis is adequate to describe the binding and the dissociation
kinetics. The values of (a) the binding rate coefficient
k
and the fractal dimension
D
f
for a
single-fractal analysis, and (b) the dissociation rate coefficient
k
d
and the fractal dimension
for dissociation
D
fd
are given in
Tables 13.3
and
13.4
.
Figure 13.3e
shows the binding and dissociation of 5.0
m
g/L of TNT in the aqueous environment
to anti-TNT mAb by a highly sensitive TNT immunosensor with a prototype fluorescence-based
detection system (KinExA Inline Biosensor, Sappidyne Instrument, Inc.;
Bromage et al.,
2007
). In this case, a single-fractal analysis is adequate to describe the binding kinetics.
