Biomedical Engineering Reference
In-Depth Information
hybridization assay when compared with the binding of the noncomplementary ODN case.
This is observed for both phases of binding, that is, for the binding rate coefficients
k
1
and
k
2
. The binding rate coefficient for the ODN-P case is 9.65 times higher than that of the bind-
ing rate coefficient for the noncomplementary ODN during the hybridization assay with EST
2-A34 reporter.
Krieg et al. (2006)
report that there is an increasing need for better sequencing methods
(
Shendure et al., 2004; Chan, 2005
). A possible alternative is sequencing by hybridization
(SBH) based on microarrays (
Hacia, 1999
).
Krieg et al. (2006)
note that new DNA sequenc-
ing techniques are being developed that use single-molecule fluorescence-based detection of
enzymatic double-strand synthesis. They point out that such applications require surface
structures on which single-stranded templates may be immobilized. Nonspecific adsorption
should be a minimum, and
Krieg et al. (2006)
report that PAC coatings are a good choice
to help minimize the nonspecific binding of the bases.
Figure 13.2a
shows the primer elongation (binding) reaction of DNA coupled directly to
PAC. A single-fractal analysis is adequate to describe the binding kinetics. The values of
the binding rate coefficient
k
and the fractal dimension
D
f
for a single-fractal analysis are
given in
Table 13.1(b)
and
13.2(b).
Figure 13.2b
shows the primer elongation (binding) reaction of DNA coupled to PAC via
biotin-streptavidin-biotin reaction. Once again, a single-fractal analysis is adequate to
describe the binding kinetics. The values of the binding rate coefficient
k
and the fractal
dimension
D
f
for a single-fractal analysis are given in
Table 13.1(b)
and
13.2(b)
. It is of inter-
est to note that as one goes from the DNA coupled directly to the PAC via the biotin-
streptavidn-biotin reaction the fractal dimension decreases by 4.22% from a value of
1400
1400
1200
1200
1000
1000
800
800
600
600
400
400
200
200
0
0
0
1000
2000
3000
4000
5000
6000
0
1000
2000
3000
4000
5000
6000
A
B
Figure 13.2
(a) Primer elongation reaction (binding) of DNA coupled directly to PAC. (b) Primer elongation
reaction (binding) of DNA coupled via biotin-streptavidin (
Krieg et al., 2006
).
Time (s)
Time (s)
