Binding of Different Analytes on Biosensor Surfaces - Biosensors and Biosensor Kinetics - page 368

Table 13.1a,b Binding and dissociation rate coefficients for (a) DNA hybridization assay with
EST2-A34 reporter ( Wang et al., 2007 ) , and (b) primer elongation of DNA coupled directly
to PAC via biotin-streptavidin-biotin (Krieg et al., 2007)

Analyte/Receptor k k 1 k 2 k d

(a) Perfectly matched ODN
(ODN-P)/EST2-A34 reporter

3.256 0.467 0.949 0.267 2.204 0.365 0.0608 0.0069

Non-complementary ODN
(ODN-N)/ES-T2-A34 reporter

0.1864 0.0705 0.0981 0.0832 3.365 0.047 na

(b) Primer elongation reaction
of DNA coupled directly to
PAC

8.822 0.424 na na na

Primer elongation reaction
of DNA coupled to PAC via
biotin-streptavidin-biotin
reaction

6.074 0.325 na na na

as the fractal dimension increases by a factor of 10.39 from a value of D f1 equal 0.212 to D f2
equal to 2.203, the binding rate coefficient increases by a factor of 34.3 from a value of k 1
equal to 0.0981 to k 2 equal to 3.365. Note, once again, that changes in the fractal dimension
or the degree of heterogeneity on the sensing surface and in the binding rate coefficient are in
the same direction. It is of interest to note that, and as may be expected, for a dual-
fractal analysis, the binding rate coefficient k is higher for the ODN-P for the complementary

Table 13.2a,b Fractal dimensions for the binding and dissociation (a) DNA hybridization assay
with EST2-A34 reporter ( Wang et al., 2007 ), and (b) primer elongation of DNA coupled directly
to PAC via biotin-streptavidin-biotin (Krieg et al., 2007)

Analyte/Receptor D f D f1 D f2 D fd

(a) Perfectly matched ODN
(ODN-P)/EST2-A34 reporter

1.0296 0.3798 0 þ 0.42434 53.15 1.16 0.0608 0.0069

Non-complementary ODN
(ODN-N)/ES-T2-A34
reporter

0.762 0.293 0.212 þ 0.491 2.2028 0.3016 na

(b) Primer elongation
reaction of DNA coupled
directly to PAC

1.8418 0.0398 na na na

Primer elongation reaction
of DNA coupled to PAC via
biotin-streptavidin-biotin
reaction

1.7642 0.0442 na na na