Biomedical Engineering Reference
In-Depth Information
CHAPTER 13
Binding of Different Analytes on
Biosensor Surfaces
Chapter Outline
13.1 Introduction
365
13.2 Theory
366
13.2.1 Single-Fractal Analysis
366
Binding Rate Coefficient
366
Dissociation Rate Coefficient
367
13.2.2 Dual-Fractal Analysis
367
Binding Rate Coefficient
367
13.3 Results
368
13.4 Conclusions
384
13.1 Introduction
Biosensor applications have expanded considerably over the last few years. Medical
applications initially dominated the biosensor market and applications. However, over the
years, the use of biosensors for the detection of analytes in other areas is gradually coming
into prominence. This is primarily due to the ease and simplicity of the use of biosensors.
In this chapter we analyze a few of the biosensor applications that have recently appeared
in the literature. The fractal analysis method will be used, as in the previous chapters, to ana-
lyze the binding and dissociation (if applicable) kinetics of a wide variety of examples avail-
able in the literature. The systems analyzed were selected at random. The analyte-receptor
systems analyzed include (a) the binding of perfectly matched oligodeoxynucleotide
(ODN-P) and noncomplementary ODN during the hybridization assay with EST2-A34
reporter (
Wang et al., 2007
), (b) binding during the primer elongation reaction of DNA cou-
pled directly to polyacrylic acid (PAC) and DNA coupled via biotin-streptavidin (
Krieg et al.,
2006
), (c) binding and dissociation of trace amounts of trinitrotoluene (TNT) in
m
g/L to anti-
TNT antibody immobilized on a prototype fluorescence-based detector system (KinExA
Inline Biosensor, Sappidyne Instrument, Inc.;
Bromage et al., 2007
), (d) binding of EBP
(estrogen binding protein) to
Saccharomyces cerevisiae
and the binding of 17
b
-estradiol
(
Baronian and Guruzada, 2007
), (e) binding of different units of restriction endonuclease
