Binding and Dissociation Kinetics of Different Analytes on Novel Biosensing Surfaces: A Fractal Analysis - Biosensors and Biosensor Kinetics - page 352

4

14

3.5

12

3

10

2.5

8

2

6

1.5

4

1

2

0.5

1 1.2 1.4 1.6 1.8 2 2.3 2.4 2.5 2.6

Fractal dimension, D f2

2.7 2.8

F G Fractal dimension, D f1

0.4 2.6

2.4

0.35

2.2

0.3

2

0.25

1.8

0.2

1.6

0.15

1.4

1.77 1.78 1.79

Fractal dimension, D fd

1.8 1.81 1.82 1.83 1.84

0 1 2 3

Con A concentration (nM)

4 5

H

I

1 10

9

0.9

8

0.8

7

0.7

6

0.6

5

0.5

4

0 1 2 3 4 5

0.5 0.6 0.7 0.8 0.9 1

J

K
Figure 12.10—cont'd
(f) Increase in the binding rate coefficient, k 1 , with an increase in the fractal dimension, D f1 .
(g) Increase in the binding rate coefficient, k 2 , with an increase in the fractal dimension, D f2 .
(h) Increase in the dissociation rate coefficient, k d , with an increase in the fractal dimension for
dissociation, D fd . (i) Decrease in the ratio of the fractal dimensions, D f2 /D f1 , with an increase in the
Con A concentration in solution. (j) Increase in the ratio of the fractal dimensions, D f1 /D fd , with an
increase in the Con A concentration in solution. (k) Increase in the affinity, K 1 (

Con A concentration (nM)

D f1 / D fd

¼ k 1 /k d ), with an

increase in the ratio of fractal dimensions, D f1 /D fd .

continued