Biomedical Engineering Reference
In-Depth Information
Table 11.4: Binding and dissociation rate coefficients for the hybridization of different analytes in solution to complementary or
noncomplementary receptors immobilized on different biosensor surfaces.
Analyte in Solution/
Receptor on surface
Biosensor Type
k
k
1
k
2
k
3
k
d
References
QCM
3,3-diaminobenzidine/
sterptavidin-peroxide
horseradish (SA-HRP)
2.7136
0.5831
1.0132
0.1069
42.513 0.141 na
na
Feng et al.
(2007
)
Electrochemical
enzymatic
genosensor
Sequence of the SARS
(severe acute respiratory
syndrome) coronavirus
(CoV) SARS-CoV/(c-DNA)
30-mer 3
0
-thiolated DNA
strand
7.0291
na
na
na
na
Abad-Valle
et al. (2007a,b
)
1.1079
Sequence
of the SARS
(severe acute respiratory
syndrome) coronavirus
(CoV) SARS-CoV/3 base
mismatch 30-mer 3
0
-
thiolated DNA strand
Electrochemical
enzymatic
genosensor
23.569
2.627 na
na
na
na
Abad-Valle
et al. (2007a,b
)
Electrochemical
detection
p-aminophenylbutyrate/
esterase 2 from
Alicyclobacillus acidocaldarius
plus oligodeoxynucleotide
(ODN) in a site-specific
manner (perfectly matched;
ODN-P)
6.9462
2.8403
37.626
1.136 na
0.07685
Wang et al.
(2007
)
1.9652
0.2542
0.00598
Electrochemical
detection
p-aminophenylbutyrate/
esterase 2 from
Alicyclobacillus acidocaldarius
plus oligodeoxynucleotide
(ODN) in a site-specific
manner
(noncomplementary ODN;
ODN-N)
0.1261
0.0409
1.5596
4.7240
na
Wang et al.
(2007
)
0.0600
0.0135
0.1142
0.4477
