Biomedical Engineering Reference
In-Depth Information
CHAPTER 11
Detection of Analytes on Arrays/
Microarrays/DNA Chips
Chapter Outline
11.1 Introduction
297
11.2 Theory
298
11.2.1 Single-Fractal Analysis
298
Binding Rate Coefficient
298
Dissociation Rate Coefficient
298
11.2.2 Dual-Fractal Analysis
299
Binding Rate Coefficient
299
11.3 Results
299
11.4 Conclusions
332
11.1 Introduction
In this chapter we use fractal analysis to analyze (a) the binding and dissociation (hybridization)
of different targets (400 nM) in solution to a probe immobilized on a DNA chip surface (
Fiche
et al., 2007
), (b) binding (hybridization) of different concentrations (in nM) of free-DNA in
solution to a 22-mer strand (bound DNA) immobilized via a phenylene-diisocyanate linker
molecule on a glass substrate (
Michel et al., 2007
), (c) binding (hybridization) of SA-HRP
(streptavidin-horseradish peroxide) in solution to a capture probe on a QCM (quartz crystal
microbalance) electrode along with a detection probe (
Feng et al., 2007
), (d) binding (hybrid-
ization) of a complementary and a noncomplementary (three-base mismatch strand) DNA in
solution to a 30-mer 3
0
-thiolated DNA strand immobilized on an electrochemical enzymatic
genosensor (
Abad-Valle et al., 2007a,b
), (e) binding (hybridization) of (i) a perfectly matched
oligonucleotide (ODN-P) and (ii) a noncomplementary ODN (ODN-N) to an electrochemical
sensor with a EST2-A34 reporter (
Wang et al., 2007
), (f) binding and dissociation during
PNA-DNA hybridization—binding of different concentrations (in
m
M) of target DNA comple-
mentary to CYP2C9*2 (target DNA2) to CYP2C9*2 as a probe PNA immobilized on a ion-
sensitive field-effect transistor (IS-FET)-based biosensor (
Uno et al., 2007
), (g) binding and
dissociation during PNA-DNA hybridization—binding of different concentrations (in
m
M) of
target DNA complementary to CYP2C9*2 (target DNA2) to CYP2C9*2 as a probe PNA
