Biomedical Engineering Reference
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Table 9.5: Binding (enrichment) and dissociation (depletion) rate coefficients of cholesterol to cyclodextrin modified HeLa cells
cultivated on a gold-coated prism ( Ziblat et al., 2006 ).
Analyte in Solution/
Cyclodextrin Modified HeLa
Cells
k
k 1
k 2
k d
k d1
k d2
20 nM m
CD cholesterol
3.883
0.668
2.913
0.211
5.3312
0.0884 1.0877
0.2302
na
na
b
20 nM m
CD cholesterol
(second cycle)
2.5156
1.100
1.1117
0.1808
6.952
0.472
na
na
na
b
10 nM m
CD dissociation
(depletion) only
na
na
na
1.954
0.211
na
na
b
10 nM m
CD dissociation
(depletion) only
b
1.3620
0.5581 1.3086
0.5019 3.1336
0.1086 0.5472
0.1052
na
na
10 mM m
b
CD-chol
1.208
0.61
0.6715
0.1529 4.1405
0.0344
na
na
na
3mM inactive analog of
m b CD, a CD
na
na
na
1.478 0.651
0.9638 0.1938 9.668 0.373
Table 9.6: Fractal dimensions for the binding (enrichment) and the dissociation (depletion) phase for cholesterol to cyclodextrin
modified HeLa cells cultivated on a gold-coated prism ( Ziblat et al., 2006 ).
Analyte in Solution/
Cyclodextrin
Modified HeLa Cells
D f
D f1
D f2
D fd
D fd1
D fd2
20 nM m
CD
2.5720
0.09632 1.892
0.1093
2.8457
0.07252 1.4094
0.2308
na
na
b
cholesterol
20 nM m b CD
cholesterol
(second cycle)
1.7630 0.4284
0 þ 0.4218
2.8392 0.1187
na
na
na
10 nM m b CD
dissociation
(depletion) only
na
na
na
1.7696 0.1055
na
na
10 nM m b CD
dissociation
(depletion) only
1.2468 0.2258
0.0002 þ 0.4752
2.1936 0.0848
1.6064 0.1521
na
na
10 mM m
CD-chol
1.78
0.3192
0.1118
þ
0.3940
2.9347
0.03426
na
na
na
b
3mM inactive analog
of m
na
na
na
1.2174
0.2366
0.4128
0.3058
2.7130
0.07898
CD,
CD
b
a
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